Millipore and Sigma-Aldrich are both brands under Merck. Nylon membranes are a hydrophilic membrane product line offered by Millipore, featuring good chemical compatibility, high mechanical strength, and a wide selection of pore sizes. They are widely used in laboratory analysis, sample preparation, organic solvent filtration, particle removal, microorganism collection, and industrial cleanliness testing.
The most notable characteristic of nylon membranes is their inherent hydrophilicity, which typically eliminates the need for additional pre-wetting when filtering aqueous samples, simplifying experimental procedures and improving operational efficiency. Additionally, nylon material exhibits good tolerance to a variety of polar organic solvents, such as acetonitrile, methanol, and tetrahydrofuran, making it highly suitable for sample filtration prior to HPLC, UHPLC, GC, and other analytical techniques.
Millipore nylon membranes can generally be divided into two main types:
Nylon Surface Membranes
Pore sizes typically range from 0.2 to 1.2 µm, suitable for fine particle removal, clarification filtration, sample preparation, and sterilization filtration applications.
Nylon Woven Grid Membranes
Mesh sizes typically range from 5 to 180 µm, suitable for large particle retention, particle counting, microscopic observation, industrial cleanliness analysis, and biological sample collection.
With their hydrophilicity, solvent resistance, and high mechanical strength, nylon membranes meet diverse filtration needs ranging from precision laboratory analysis to industrial particle monitoring.
Nylon membranes are inherently hydrophilic and can generally be used directly for aqueous sample filtration without the need for alcohol pre-wetting, making operations more convenient compared to hydrophobic membrane materials.
Nylon material exhibits good tolerance to various organic solvents and aqueous solutions, making it suitable for filtration of polar organic phases such as acetonitrile, methanol, and tetrahydrofuran. It is commonly used for sample preparation prior to chromatographic analysis.
Product specifications cover pore sizes from 0.22 µm to 20 µm, and even larger mesh sizes, accommodating applications including sterilization filtration, particle removal, clarification filtration, large particle pre-filtration, and particle analysis.
Nylon membranes offer good toughness and mechanical strength, making them resistant to damage during routine vacuum filtration, pressure filtration, and sample preparation processes, and suitable for high-frequency laboratory use.
Nylon membranes can withstand maximum operating temperatures up to 100 °C. It should be noted that when operating temperatures exceed 75 °C, the membrane may exhibit some curling; therefore, compatibility testing is recommended for high-temperature applications.
| Material | Thickness | Model | Pore Size | Available Diameters | Catalog Number | Pack Size | Recommended Applications |
|---|---|---|---|---|---|---|---|
| Nylon | 175 µm | GNWP | 0.22 µm | 13, 25, 47, 90 mm | GNWP01300 / GNWP02500 / GNWP04700 / GNWP09025 | 13/25/47 mm: 100/pk; 90 mm: 25/pk | Polar organic solvent filtration, high mechanical strength filtration, fine particle removal, sterilization filtration scenarios |
| Nylon | 175 µm | HNWP | 0.45 µm | 13, 25, 47, 90 mm | HNWP01300 / HNWP02500 / HNWP04700 / HNWP09025 | 13/25/47 mm: 100/pk; 90 mm: 25/pk | Polar organic solvent filtration, sample clarification, routine particle removal, high mechanical strength filtration |
| Nylon | 175 µm | NY200 | 20 µm | 47 mm | NY2004700 | 100/pk | Large particle filtration, organic solvent pre-filtration, coarse particle retention, sample pretreatment |
Nylon membranes offer good compatibility with a variety of polar organic solvents and can be used for filtration of organic phases such as acetonitrile, methanol, and tetrahydrofuran. For HPLC, UHPLC, and other analytical techniques, using nylon membranes for sample preparation can effectively remove particulate impurities, reduce the risk of instrument clogging, and improve analytical stability.
Applicable scenarios include:
HPLC mobile phase filtration
Organic solvent clarification
Chromatographic sample preparation
Laboratory analytical sample filtration
Particle removal from polar organic solutions
Nylon membranes are hydrophilic and suitable for direct filtration of aqueous samples. The 0.45 µm pore size is commonly used for routine particle removal and sample clarification, while 0.22 µm is suitable for finer particle retention and sterilization filtration scenarios.
Applicable samples include:
Water samples
Buffers
Bioanalytical samples
Laboratory-prepared solutions
Mixed aqueous/organic phase samples
0.22 µm pore size nylon surface membranes can be used for sterilization filtration in bioanalytical, aqueous, and some organic phase applications. For aseptic filtration, it is recommended to evaluate based on sample properties, filtration equipment, operating environment, and experimental validation results.
Applicable scenarios include:
Biological sample preparation
Aqueous solution sterilization filtration
Some organic phase sterilization filtration
Laboratory microbiological control filtration
Nylon woven grid membranes are suitable for surface particle retention, as particles do not penetrate deeply into the membrane structure, facilitating subsequent microscopic observation, particle counting, and image analysis. The 5 µm woven grid membrane is commonly used for cleanliness testing in automotive, mechanical manufacturing, and precision component industries.
Applicable scenarios include:
Automotive component cleanliness testing
Precision manufacturing particle residue analysis
Industrial rinse fluid particle collection
Microscopic particle observation
Particle imaging system background filtration
Nylon membranes can be used for collecting algae, cells, and microorganisms from water or biological samples. Based on the target sample size, different pore sizes or mesh specifications can be selected to achieve effective retention and subsequent observation/analysis.
Applicable scenarios include:
Algae collection
Cell collection
Microbial enrichment
Environmental water sample analysis
Biological sample pretreatment
Nylon membranes can also be used for sample filtration, sorting, and transfer in model organism experiments involving C. elegans and zebrafish, suitable for certain drug screening and toxicology research workflows.
Applicable scenarios include:
C. elegans sorting
Zebrafish drug screening
Toxicology experimental sample processing
Model organism sample separation
| Application Requirement | Recommended Pore Size |
|---|---|
| Sterilization filtration, fine particle removal | 0.22 µm |
| Routine sample clarification, HPLC sample filtration | 0.45 µm |
| Large particle filtration, pre-filtration | 20 µm |
| Industrial cleanliness particle analysis | 5 µm or other woven grid specifications |
| Algae, cells, large-particle biological sample collection | Based on target particle/cell size |
| Sample Type | Recommended Choice |
|---|---|
| Aqueous samples | GNWP 0.22 µm or HNWP 0.45 µm |
| Polar organic solvents | GNWP / HNWP nylon membranes |
| High-particle-load samples | Pre-filter with NY200 20 µm, then fine-filter with 0.45 µm or 0.22 µm |
| Particle observation and counting required | Nylon woven grid membrane |
| Sterilization filtration required | GNWP 0.22 µm |
| Diameter | Applicable Scenarios |
|---|---|
| 13 mm | Small-volume sample filtration, suitable for small-scale sample preparation |
| 25 mm | Routine laboratory sample filtration, compatible with syringe filters or small filter holders |
| 47 mm | Vacuum filtration, solvent filtration, larger-volume sample processing |
| 90 mm | Large-volume sample filtration or industrial laboratory applications |
Before use, select the appropriate membrane pore size and diameter based on sample type, filtration objective, and filtration volume. Confirm that the membrane packaging is intact, free from damage, contamination, or moisture.
Recommended tools and supplies:
Nylon membrane
Filter holder or membrane clamp
Vacuum filtration apparatus or pressure filtration device
Forceps
Receiving flask or collection container
Sample to be filtered
Necessary personal protective equipment
Use clean forceps to handle the membrane, avoiding direct hand contact with the effective filtration area. Place the membrane flat onto the filter support surface, ensuring it is free from folds, lifted edges, or damage.
Installation notes:
Membrane must completely cover the filtration area
Edges should be securely pressed to prevent leakage
Do not over-stretch or bend the membrane
For precision analysis, avoid dust and particle contamination
Add the sample to the filter apparatus and apply vacuum or pressure gradually to allow the sample to pass through the membrane.
Operational recommendations:
Pre-filter or coarse-filter high-particle samples first
Avoid applying high pressure at the start
Replace the membrane if flow rate decreases significantly
For valuable samples, rinse the filtration system with a small volume of the sample first
For organic solvents, verify compatibility of the filter holder and seals with the solvent
After filtration, choose the appropriate next step based on the experimental objective:
If the filtrate is the target: collect filtrate for subsequent analysis
If particles are the target: remove the membrane for microscopic observation, weighing, imaging, or particle counting
If microorganisms or cells are the target: transfer the membrane to culture media, glass slides, or other analysis systems
Used membranes should be disposed of according to sample properties. If filtering toxic substances, organic solvents, corrosive solutions, or biological samples, follow laboratory safety regulations and local disposal requirements.
Although nylon membranes offer good solvent resistance, compatibility testing is recommended before use with strong acids, strong bases, or specialty solvents.
For high-temperature filtration, note that membranes may curl, especially when temperatures exceed 75 °C.
High-particle-load samples should not be filtered directly through 0.22 µm membranes; pre-filtration with 20 µm or larger pore size membranes is recommended.
For HPLC or UHPLC analysis, ensure that the membrane does not cause significant adsorption or interference with target analytes.
For sterilization filtration applications, evaluate based on aseptic technique, filtration equipment, and experimental validation results.
Membranes are consumable products and are generally not recommended for reuse.
When using forceps, handle the membrane by the edge to avoid contaminating the effective filtration surface.
Q1: Are nylon membranes suitable for organic solvent filtration?
A: Yes. Nylon membranes offer good tolerance to a variety of polar organic solvents, such as acetonitrile, methanol, and tetrahydrofuran, and are commonly used for HPLC mobile phase filtration and organic phase sample preparation.
Q2: Can nylon membranes filter aqueous solutions?
A: Yes. Nylon membranes are hydrophilic and typically do not require pre-wetting for aqueous sample filtration. They can be used directly for aqueous solution clarification, particle removal, and sample preparation.
Q3: How do I choose between 0.22 µm and 0.45 µm?
A: Choose 0.22 µm for finer particle removal or sterilization filtration applications; choose 0.45 µm for routine sample clarification, HPLC sample filtration, or general particle removal.
Q4: Can high-particle samples be filtered directly through 0.22 µm membranes?
A: Not recommended. High-particle samples tend to clog small-pore membranes. Pre-filtration with 20 µm or other large-pore membranes is recommended before fine filtration with 0.45 µm or 0.22 µm.
Q5: What is the primary use of NY200 20 µm membranes?
A: NY200 20 µm nylon membranes are primarily used for large particle filtration, organic solvent pre-filtration, and coarse particle retention, serving as a pretreatment step before fine filtration.
Q6: Do nylon membranes require pre-wetting?
A: Pre-wetting is generally not required for aqueous samples because nylon membranes are inherently hydrophilic. However, for specialty solvent systems, low-surface-tension samples, or high-precision analysis, pretreatment may be performed according to protocol requirements.
Q7: What is the maximum temperature nylon membranes can withstand?
A: Nylon membranes can withstand maximum operating temperatures up to 100 °C. However, curling may occur above 75 °C, so testing is recommended before high-temperature use.
Q8: Are nylon membranes suitable for particle counting?
A: Yes. In particular, nylon woven grid membranes feature a surface retention structure that allows particles to remain on the membrane surface, facilitating subsequent microscopic observation, particle counting, and image analysis.
Q9: Can nylon membranes be used for biological sample filtration?
A: Yes. Nylon membranes can be used for collecting cells, algae, and microorganisms from certain biological samples, as well as for clarification and sterilization filtration of bioanalytical samples. Specific selection should be based on sample properties and target particle size.
Q10: What is the difference between GNWP and HNWP?
A: GNWP typically corresponds to 0.22 µm pore size, suitable for fine particle removal and sterilization filtration; HNWP typically corresponds to 0.45 µm pore size, suitable for routine particle removal, sample clarification, and chromatographic sample preparation.
Millipore / Sigma-Aldrich Nylon membranes are laboratory filtration consumables that combine hydrophilicity, solvent resistance, and high mechanical strength. Their pore size specifications cover 0.22 µm, 0.45 µm, 20 µm, and larger woven grid membranes, meeting diverse needs from sterilization filtration, sample clarification, and organic solvent filtration to industrial particle analysis and biological sample collection.
If your experiments involve polar organic solvents, aqueous samples, particle removal, HPLC sample preparation, or industrial cleanliness analysis, nylon membranes offer a stable, versatile, and easy-to-use choice.
For fine filtration, choose GNWP 0.22 µm; for routine clarification filtration, choose HNWP 0.45 µm; for large particle pre-filtration, choose NY200 20 µm. Selecting the appropriate membrane specification based on sample properties, filtration objectives, and processing volume will effectively improve filtration efficiency and experimental result stability.
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| Model / Pore Size | Φ13mm, 100/pk | Φ25mm, 100/pk | Φ47mm, 100/pk | Φ90mm, 25/pk |
|---|---|---|---|---|
| GNWP / 0.22μm | GNWP01300 Inquiry | GNWP02500 $250 | GNWP04700 $317 | GNWP09025 Inquiry |
| HNWP / 0.45μm | HNWP01300 Inquiry | HNWP02500 $312 | HNWP04700 $331 | HNWP09025 Inquiry |
| NY200 / 20μm | — | — | NY2004700 $341 | — |
Partial references citing our materials (from Google Scholar)
Carbon Dioxide Reduction
1. ACS Nano Strain Relaxation in Metal Alloy Catalysts Steers the Product Selectivity of Electrocatalytic CO2 Reduction
The bipolar membrane (Fumasep FBM) in this paper was purchased from SCI Materials Hub, which was used in rechargeable Zn-CO2 battery tests. The authors reported a strain relaxation strategy to determine lattice strains in bimetal MNi alloys (M = Pd, Ag, and Au) and realized an outstanding CO2-to-CO Faradaic efficiency of 96.6% with outstanding activity and durability toward a Zn-CO2 battery.
2. Front. Chem. Boosting Electrochemical Carbon Dioxide Reduction on Atomically Dispersed Nickel Catalyst
In this paper, Vulcan XC-72R was purchased from SCI Materials Hub. Vulcan XC 72R carbon is the most common catalyst support used in the anode and cathode electrodes of Polymer Electrolyte Membrane Fuel Cells (PEMFC), Direct Methanol Fuel Cells (DMFC), Alkaline Fuel Cells (AFC), Microbial Fuel Cells (MFC), Phosphoric Acid Fuel Cells (PAFC), and many more!
3. Adv. Mater. Partially Nitrided Ni Nanoclusters Achieve Energy-Efficient Electrocatalytic CO2 Reduction to CO at Ultralow Overpotential
An AEM membrane (Sustainion X37-50 Grade RT, purchased from SCI Materials Hub) was activated in 1 M KOH for 24 h, washed with ultra-purity water prior to use.
4. Adv. Funct. Mater. Nanoconfined Molecular Catalysts in Integrated Gas Diffusion Electrodes for High-Current-Density CO2 Electroreduction
In this paper (Supporting Information), an anion exchanged membrane (Fumasep FAB-PK-130 obtained from SCI Materials Hub (www.scimaterials.cn)) was used to separate the catholyte and anolyte chambers.
SCI Materials Hub: we also recommend our Fumasep FAB-PK-75 for the use in a flow cell.
5. Appl. Catal. B Efficient utilization of nickel single atoms for CO2 electroreduction by constructing 3D interconnected nitrogen-doped carbon tube network
In this paper, the Nafion 117 membrane was obtained from SCI Materials Hub.
In this paper, Proton exchange membrane (Nafion 117), Nafion D520, and Toray 060 carbon paper were purchased from SCI Materials Hub.
7. National Science Review Confinement of ionomer for electrocatalytic CO2 reduction reaction via efficient mass transfer pathways
An anion exchange membrane (PiperION-A15-HCO3) was obtained from SCI Materials Hub.
8. Catalysis Communications Facilitating CO2 electroreduction to C2H4 through facile regulating {100} & {111} grain boundary of Cu2O
Carbon paper (TGPH060), membrane solution (Nafion D520), and ionic membrane (Nafion N117) were obtained from Wuhu Eryi Material Technology Co., Ltd (a company under SCI Materials Hub).
Batteries
1. J. Mater. Chem. A Blocking polysulfides with a Janus Fe3C/N-CNF@RGO electrode via physiochemical confinement and catalytic conversion for high-performance lithium–sulfur batteries
Graphene oxide (GO) in this paper was obtained from SCI Materials Hub. The authors introduced a Janus Fe3C/N-CNF@RGO electrode consisting of 1D Fe3C decorated N-doped carbon nanofibers (Fe3C/N-CNFs) side and 2D reduced graphene oxide (RGO) side as the free-standing carrier of Li2S6 catholyte to improve the overall electrochemical performance of Li-S batteries.
This paper used more than 10 kinds of materials from SCI Materials Hub and the authors gave detailed properity comparsion.
The commercial IEMs of Fumasep FAB-PK-130 and Nafion N117 were obtained from SCI Materials Hub.
Gas diffusion layers of GDL340 (CeTech) and SGL39BC (Sigracet) and Nafion dispersion (Nafion D520) were obtained from SCI Materials Hub.
Zn foil (100 mm thickness) and Zn powder were obtained from the SCI Materials Hub.
Commercial 20% Pt/C, 40% Pt/C and IrO2 catalysts were also obtained from SCI Materials Hub.
3. Journal of Energy Chemistry Vanadium oxide nanospheres encapsulated in N-doped carbon nanofibers with morphology and defect dual-engineering toward advanced aqueous zinc-ion batteries
In this paper, carbon cloth (W0S1011) was obtained from SCI Materials Hub. The flexible carbon cloth matrix guaranteed the stabilization of the electrode and improved the conductivity of the cathode.
4. Energy Storage Materials Defect-abundant commercializable 3D carbon papers for fabricating composite Li anode with high loading and long life
The 3D carbon paper (TGPH060 raw paper) were purchased from SCI Materials Hub.
5. Nanomaterials A Stable Rechargeable Aqueous Zn–Air Battery Enabled by Heterogeneous MoS2 Cathode Catalysts
Nafion D520 (5 wt%), and carbon paper (GDL340) were received from SCI-Materials-Hub.
Carbon cloth (W0S1011) and other electrochemical consumables required for air cathode were provided by SCI Materials Hub.
Oxygen Reduction Reaction
1. J. Chem. Eng. Superior Efficiency Hydrogen Peroxide Production in Acidic Media through Epoxy Group Adjacent to Co-O/C Active Centers on Carbon Black
In this paper, Vulcan XC 72 carbon black, ion membrane (Nafion N115, 127 μL), Nafion solution (D520, 5 wt%), and carbon paper (AvCarb GDS 2230 and Spectracarb 2050A-1050) were purchased from SCI Materials Hub.
2. Journal of Colloid and Interface Science Gaining insight into the impact of electronic property and interface electrostatic field on ORR kinetics in alloy engineering via theoretical prognostication and experimental validation
The 20 wt% Pt3M (M = Cr, Co, Cu, Pd, Sn, and Ir) were purchased from SCI Materials Hub. This work places emphasis on the kinetics of the ORR concerning Pt3M (M = Cr, Co, Cu, Pd, Sn, and Ir) catalysts, and integrates theoretical prognostication and experimental validation to illuminate the fundamental principles of alloy engineering.
Water Electrolysis
1. International Journal of Hydrogen Energy Gold as an efficient hydrogen isotope separation catalyst in proton exchange membrane water electrolysis
The cathodic catalysts of Pt/C (20 wt%, 2–3 nm) and Au/C (20 wt%, 4–5 nm) were purchased from SCI Materials Hub.
2. Small Science Silver Compositing Boosts Water Electrolysis Activity and Durability of RuO2 in a Proton-Exchange-Membrane Water Electrolyzer
Two fiber felts (0.35 mm thickness, SCI Materials Hub) were used as the porous transport layers at both the cathode and the anode.
3. Advanced Functional Materials Hierarchical Crystalline/Amorphous Heterostructure MoNi/NiMoOx for Electrochemical Hydrogen Evolution with Industry-Level Activity and Stability
Anion-exchange membrane (FAA-3-PK-130) was obtained from SCI Materials Hub website.
Fuel Cells
1. Polymer Sub-two-micron ultrathin proton exchange membrane with reinforced mechanical strength
Gas diffusion electrode (60% Pt/C, Carbon paper) was purchased from SCI Materials Hub.
Characterization
1. Chemical Engineering Journal Electrochemical reconstitution of Prussian blue analogue for coupling furfural electro-oxidation with photo-assisted hydrogen evolution reaction
An Au nanoparticle film was deposited on the total reflecting plane of a single reflection ATR crystal (SCI Materials Hub, Wuhu, China) via sputter coater.
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